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KMID : 1145120180160030427
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Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 3 p.427 ~ p.435
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Effects of Coptis chinensis Extracts on Matrix Metalloproteinase-1 Suppression through JNK/AP-1 Axis in Human Dermal Fibroblasts
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Kim Ji-Yea
Bae Seung-Hee
Jung Jin-Hyuk
An Sung-Kwan
An In-Sook
Kim Young-Sam
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Abstract
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Purpose: In this study, we investigate the effect of Coptis chinensis extract (CCE) on matrix metalloprotease 1 (MMP1 ) expression in dermal fibroblasts.
Method: Human dermal fibroblasts (HDFs) were used to assess the effect of CCE on oxidative stress-induced MMP1 expression. A water-soluble tetrazolium salt (WST-1) assay was conducted to determine the cytotoxicity of CCE in HDFs. To investigate whether CCE has a protective effect on oxidative stress-induced MMP1 downregulation, HDFs were pretreated with CCE (2.5 and 5 μg/mL) for 9 h, and then with 200 μM H2O2 for 3 h. Total RNAs were collected and the level of MMP1 expression was determined using quantitative real-time polymerase chain reaction (qRT-PCR) assay with its specific primers. Also, luciferase reporter gene assay for activator protein-1 (AP-1) activity was performed with AP-1 luciferase reporter gene constructs transfected into HDFs. Furthermore, c-Jun N-terminal kinase (JNK) and p-JNK protein levels were detected by western blot analysis.
Results: Lower concentration of CCE (<5 μg/mL) showed little cytotoxicity in HDFs. Pre-treatment of the cells with CCE significantly inhibited H2O2-induced MMP1 downregulation. Further experiments revealed that CCE-mediated MMP1 downregulated was mediated via suppression of AP-1 transcriptional activity. Moreover, western blot analysis revealed that CCE inhibited the phosphorylation and activation of JNK, which is known to enhancing AP-1 activation. Therefore, these results indicate that CCE regulates MMP1 expression via JNK/AP-1 signaling pathway in HDFs.
Conclusion: These results in this paper show that CCE has a protective effect on oxidative stress-induced MMP1 expression in HDFS and may uses as a possible reagent on anti-aging cosmetics.
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KEYWORD
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Coptis chinensis, Dermal fibroblasts, Matrix metalloproteinase, Oxidative stress, JNK/AP-1 signaling pathway
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